World supplier of antibodies, antigens and kits since 2007
Primary Antibodies

Smad3 (Phospho-Thr179) Antibody

E11-1003ACite ID: EN0017829DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
P84022
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names JV15-2, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, Smad 3
All Sites Human: Thr179; Mouse: Thr179; Rat: Thr179
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of threonine 179 (P-E-TP-P-P).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity Smad3 (Phospho-Thr179) antibody detects endogenous levels of Smad3 only when phosphorylated at threonine 179.
ReferencesZhang Y., Nature 383:168-172(1996).
Riggins G.J., Nat. Genet. 13:347-349(1996).
Arai T., Cancer Lett. 122:157-163(1998).
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemistry analysis of paraffin-embedded human thymus gland tissue using Smad3 (Phospho- Thr179) antibody.
Immunohistochemistry analysis of paraffin-embedded human thymus gland tissue using Smad3 (Phospho- Thr179) antibody.
Western blot analysis of extracts from HeLa cells, treated with TNF (20ng/ml, 2mins), using Smad3 (Phospho- Thr179) antibody.
Western blot analysis of extracts from HeLa cells, treated with TNF (20ng/ml, 2mins), using Smad3 (Phospho- Thr179) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-1003A-Conjugate. Price: $485 / Size: 100μl.

E11-1003A-FITC$485 / 100μlE11-1003A-Biotin$485 / 100μlE11-1003A-HRP$485 / 100μlE11-1003A-AP$485 / 100μlE11-1003A-AF350$485 / 100μlE11-1003A-AF405M$485 / 100μlE11-1003A-AF488$485 / 100μlE11-1003A-AF514$485 / 100μlE11-1003A-AF532$485 / 100μlE11-1003A-AF555$485 / 100μlE11-1003A-AF568$485 / 100μlE11-1003A-AF405S$485 / 100μlE11-1003A-AF405L$485 / 100μlE11-1003A-AF546$485 / 100μlE11-1003A-AF594$485 / 100μlE11-1003A-AF610$485 / 100μlE11-1003A-AF635$485 / 100μlE11-1003A-AF647$485 / 100μlE11-1003A-AF680$485 / 100μlE11-1003A-AF700$485 / 100μlE11-1003A-AF750$485 / 100μlE11-1003A-AF790$485 / 100μlE11-1003A-APC$485 / 100μlE11-1003A-PE$485 / 100μlE11-1003A-Cy3$485 / 100μlE11-1003A-Cy5$485 / 100μlE11-1003A-Cy5.5$485 / 100μlE11-1003A-Cy7$485 / 100μlE11-1003A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.