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Primary Antibodies

Smad2/3 (Phospho-Thr8) Antibody

E11-1001ACite ID: EN0017819DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 ELISA: 1:10000
P84022
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names JV15-2, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, Smad 3; JV18-1, MADH2, MADR2, Mad-related protein 2, Mothers against DPP homolog 2, Mothers against decapentaplegic homolog 2, Smad 2
All Sites Human: Thr8; Mouse: Thr8; Rat: Thr8
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Smad2/3 around the phosphorylation site of threonine 8(P-F-TP-P-P).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity Smad2/3 (Phospho-Thr8) antibody detects endogenous levels of Smad2/3 only when phosphorylated at threonine 8.
ReferencesZhang Y., Nature 383:168-172(1996).
Riggins G.J., Nat. Genet. 13:347-349(1996).
Arai T., Cancer Lett. 122:157-163(1998).
Price225$
Western blot analysis of extracts from RAW264.7 cells, using Smad2/3 (Phospho-Thr8) antibody.
Western blot analysis of extracts from RAW264.7 cells, using Smad2/3 (Phospho-Thr8) antibody.
📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E11-1001A-Conjugate. Price: $485 / Size: 100μl.

E11-1001A-FITC$485 / 100μlE11-1001A-Biotin$485 / 100μlE11-1001A-HRP$485 / 100μlE11-1001A-AP$485 / 100μlE11-1001A-AF350$485 / 100μlE11-1001A-AF405M$485 / 100μlE11-1001A-AF488$485 / 100μlE11-1001A-AF514$485 / 100μlE11-1001A-AF532$485 / 100μlE11-1001A-AF555$485 / 100μlE11-1001A-AF568$485 / 100μlE11-1001A-AF405S$485 / 100μlE11-1001A-AF405L$485 / 100μlE11-1001A-AF546$485 / 100μlE11-1001A-AF594$485 / 100μlE11-1001A-AF610$485 / 100μlE11-1001A-AF635$485 / 100μlE11-1001A-AF647$485 / 100μlE11-1001A-AF680$485 / 100μlE11-1001A-AF700$485 / 100μlE11-1001A-AF750$485 / 100μlE11-1001A-AF790$485 / 100μlE11-1001A-APC$485 / 100μlE11-1001A-PE$485 / 100μlE11-1001A-Cy3$485 / 100μlE11-1001A-Cy5$485 / 100μlE11-1001A-Cy5.5$485 / 100μlE11-1001A-Cy7$485 / 100μlE11-1001A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.

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