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Primary Antibodies
Smad2 (Phospho-Ser250) Antibody
Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)
WB: 1:500~1:1000 ELISA: 1:10000
$225/ 100μg
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Other Names JV18-1; Mad-related protein 2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; Mothers against DPP homolog 2; Smad 2
All Sites Human: Ser250; Mouse: Ser250; Rat: Ser250
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Smad2 around the phosphorylation site of serine 250 (E-L-SP-P-T).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Smad2 (Phospho-Ser250) antibody detects endogenous levels of Smad2 only when phosphorylated at serine 250.
ReferencesRiggins G.J., Nat. Genet. 13-347-349(1996).
Zhang Y., Nature 383-168-172(1996).
Eppert K., Cell 86:543-552(1996).
Zhang Y., Nature 383-168-172(1996).
Eppert K., Cell 86:543-552(1996).
Price225$
Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using Smad2 (Phospho- Ser250) antibody.
Conjugated Antibody
This product is also available conjugated. Format: E11-0998A-Conjugate. Price: $485 / Size: 100μl.
E11-0998A-FITC$485 / 100μlE11-0998A-Biotin$485 / 100μlE11-0998A-HRP$485 / 100μlE11-0998A-AP$485 / 100μlE11-0998A-AF350$485 / 100μlE11-0998A-AF405M$485 / 100μlE11-0998A-AF488$485 / 100μlE11-0998A-AF514$485 / 100μlE11-0998A-AF532$485 / 100μlE11-0998A-AF555$485 / 100μlE11-0998A-AF568$485 / 100μlE11-0998A-AF405S$485 / 100μlE11-0998A-AF405L$485 / 100μlE11-0998A-AF546$485 / 100μlE11-0998A-AF594$485 / 100μlE11-0998A-AF610$485 / 100μlE11-0998A-AF635$485 / 100μlE11-0998A-AF647$485 / 100μlE11-0998A-AF680$485 / 100μlE11-0998A-AF700$485 / 100μlE11-0998A-AF750$485 / 100μlE11-0998A-AF790$485 / 100μlE11-0998A-APC$485 / 100μlE11-0998A-PE$485 / 100μlE11-0998A-Cy3$485 / 100μlE11-0998A-Cy5$485 / 100μlE11-0998A-Cy5.5$485 / 100μlE11-0998A-Cy7$485 / 100μlE11-0998A-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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