Other Names REPA2, RF-A, RP-A, RPA2, RPA32, replication factor-A protein 2, replication protein A 32 kDa subunit, replication protein A2
All Sites Human: Thr21; Mouse: Thr21
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human RFA2 around the phosphorylation site of threonine 21 (G-Y-TP-Q-S).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity RFA2 (Phospho-Thr21) antibody detects endogenous levels of RFA2 only when phosphorylated at threonine 21.
ReferencesErdile L.F., J. Biol. Chem. 265:3177-3182(1990).
Gregory S.G., Nature 441:315-321(2006).
Niu H., J. Biol. Chem. 272:12634-12641(1997).
Cho J.M., Nucleic Acids Res. 28:3478-3485(2000).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells