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Primary Antibodies

RFA2 (Phospho-Thr21) Antibody

E11-0985ACite ID: EN0017800DatasheetCOAMSDS
Human, Mouse
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
P15927
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names REPA2, RF-A, RP-A, RPA2, RPA32, replication factor-A protein 2, replication protein A 32 kDa subunit, replication protein A2
All Sites Human: Thr21; Mouse: Thr21
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human RFA2 around the phosphorylation site of threonine 21 (G-Y-TP-Q-S).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity RFA2 (Phospho-Thr21) antibody detects endogenous levels of RFA2 only when phosphorylated at threonine 21.
ReferencesErdile L.F., J. Biol. Chem. 265:3177-3182(1990).
Gregory S.G., Nature 441:315-321(2006).
Niu H., J. Biol. Chem. 272:12634-12641(1997).
Cho J.M., Nucleic Acids Res. 28:3478-3485(2000).
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from HeLa cells, treated with Adriamycin (0.5ug/ml, 24hours), using RFA2 (Phospho-Thr21) antibody.
Western blot analysis of extracts from HeLa cells, treated with Adriamycin (0.5ug/ml, 24hours), using RFA2 (Phospho-Thr21) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0985A-Conjugate. Price: $485 / Size: 100μl.

E11-0985A-FITC$485 / 100μlE11-0985A-Biotin$485 / 100μlE11-0985A-HRP$485 / 100μlE11-0985A-AP$485 / 100μlE11-0985A-AF350$485 / 100μlE11-0985A-AF405M$485 / 100μlE11-0985A-AF488$485 / 100μlE11-0985A-AF514$485 / 100μlE11-0985A-AF532$485 / 100μlE11-0985A-AF555$485 / 100μlE11-0985A-AF568$485 / 100μlE11-0985A-AF405S$485 / 100μlE11-0985A-AF405L$485 / 100μlE11-0985A-AF546$485 / 100μlE11-0985A-AF594$485 / 100μlE11-0985A-AF610$485 / 100μlE11-0985A-AF635$485 / 100μlE11-0985A-AF647$485 / 100μlE11-0985A-AF680$485 / 100μlE11-0985A-AF700$485 / 100μlE11-0985A-AF750$485 / 100μlE11-0985A-AF790$485 / 100μlE11-0985A-APC$485 / 100μlE11-0985A-PE$485 / 100μlE11-0985A-Cy3$485 / 100μlE11-0985A-Cy5$485 / 100μlE11-0985A-Cy5.5$485 / 100μlE11-0985A-Cy7$485 / 100μlE11-0985A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.