Other Names P105-RB, PP105, PP110, RB-1, RB1, Retinoblastoma-associated protein
All Sites Human: Thr826; Mouse: Thr817; Rat: Thr797
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Retinoblastoma around the phosphorylation site of threonine 826 (K-M-TP-P-R).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Retinoblastoma (Phospho-Thr826) antibody detects endogenous levels of Retinoblastoma only when phosphorylated at threonine 826.
ReferencesLee W.-H., Nature 329:642-645(1987).
Friend S.H., Proc. Natl. Acad. Sci. U.S.A. 84:9059-9063(1987).
McGee T.L., Gene 80:119-128(1989).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells