Other Names P105-RB, PP105, PP110, RB-1, RB1, Retinoblastoma-associated protein
All Sites Human: Ser249; Mouse: Ser243; Rat: Ser221
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Retinoblastoma around the phosphorylation site of serine 249 (N-G-SP-P-R).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using
epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Retinoblastoma (Phospho-Ser249) antibody detects endogenous levels of Retinoblastoma only when phosphorylated at serine 249.
ReferencesIshizaka Y., Oncogene 4:789-794(1989).
Angrist M., Hum. Mol. Genet. 4:821-830(1995).
Sjoeblom T., Science 314:268-274(2006).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells