Other Names CDKN1B, CDN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, KIP1
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human p27 Kip1 around the phosphorylation site of threonine 198 (R-R-R-Q-TP).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity p27 Kip1 (Phospho-Thr198) antibody detects endogenous levels of p27 Kip1 only when phosphorylated at threonine 198.
ReferencesPolyak K., Cell 78:59-66(1994).
Pietenpol J.A., Cancer Res. 55:1206-1210(1995).
Porter L.A., Mol. Biol. Cell 14:3664-3674(2003).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells