Other Names H4F2, HIST, HIST1H4A, Histone H4
All Sites Human: Ser47; Mouse: Ser47
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Histone H4 around the phosphorylation site of serine 47 (R-I-SP-G-L).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the
phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Histone H4 (Phospho-Ser47) antibody detects endogenous levels of Histone H4 only when phosphorylated at serine 47.
ReferencesElisheva Smith, Nucleic Acids Res., Mar 2005; 33: 1298 - 1308.
Lynn Glowczewski, Mol. Cell. Biol., Dec 2004; 24: 10180 - 10192.
Partha Mitra, Mol. Cell. Biol., Nov 2003; 23: 8110 - 8123.
S Y Roth, Genes & Dev., Mar 1992; 6: 411 - 425.