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Primary Antibodies

HER3 (Phospho-Tyr1222) Antibody

E11-0944ACite ID: EN0017757DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000
P21860
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names EC 2.7.10.1, ERB3, ERBB3, HER3, Receptor protein-tyrosine kinase erbB-3 precursor, Tyrosine kinase-type cell surface receptor HER3, c-erbB3, kinase ErbB3
All Sites Human: Tyr1222; Mouse: Tyr1219; Rat: Tyr1219
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human HER3/ErbB3 around the phosphorylation site of tyrosine 1222 (L-G-YP-E-Y).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity HER3/ErbB3 (Phospho-Tyr1222) antibody detects endogenous levels of HER3/ErbB3 only when phosphorylated at tyrosine 1222.
ReferencesKatoh M., Biochem. Biophys. Res. Commun. 192:1189-1197(1993).
Kinugasa Y., Biochem. Biophys. Res. Commun. 321:1045-1049(2004).
Narkis G., Am. J. Hum. Genet. 81:589-595(2007).
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from HUVEC cells, treated with EGF (200ng/ml, 30mins), using HER3/ErbB3 (Phospho-Tyr1222) antibody.
Western blot analysis of extracts from HUVEC cells, treated with EGF (200ng/ml, 30mins), using HER3/ErbB3 (Phospho-Tyr1222) antibody.
Immunofluorescence analysis of HuvEc cells, using HER3/ErbB3 (Phospho-Tyr1222) antibody.
Immunofluorescence analysis of HuvEc cells, using HER3/ErbB3 (Phospho-Tyr1222) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0944A-Conjugate. Price: $485 / Size: 100μl.

E11-0944A-FITC$485 / 100μlE11-0944A-Biotin$485 / 100μlE11-0944A-HRP$485 / 100μlE11-0944A-AP$485 / 100μlE11-0944A-AF350$485 / 100μlE11-0944A-AF405M$485 / 100μlE11-0944A-AF488$485 / 100μlE11-0944A-AF514$485 / 100μlE11-0944A-AF532$485 / 100μlE11-0944A-AF555$485 / 100μlE11-0944A-AF568$485 / 100μlE11-0944A-AF405S$485 / 100μlE11-0944A-AF405L$485 / 100μlE11-0944A-AF546$485 / 100μlE11-0944A-AF594$485 / 100μlE11-0944A-AF610$485 / 100μlE11-0944A-AF635$485 / 100μlE11-0944A-AF647$485 / 100μlE11-0944A-AF680$485 / 100μlE11-0944A-AF700$485 / 100μlE11-0944A-AF750$485 / 100μlE11-0944A-AF790$485 / 100μlE11-0944A-APC$485 / 100μlE11-0944A-PE$485 / 100μlE11-0944A-Cy3$485 / 100μlE11-0944A-Cy5$485 / 100μlE11-0944A-Cy5.5$485 / 100μlE11-0944A-Cy7$485 / 100μlE11-0944A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.