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Primary Antibodies

HDAC6 (Phospho-Ser22) Antibody

E11-0941ACite ID: EN0017753DatasheetCOAMSDS
Human, Mouse
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000
Q9UBN7
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names HD6, HDA6, Histone deacetylase 6, JM21
All Sites Human: Ser22; Mouse: Ser21
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human HDAC6 around the phosphorylation site of serine 22 (P-Q-SP-P-P).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity HDAC6 (Phospho-Ser22) antibody detects endogenous levels of HDAC6 only when phosphorylated at serine 22.
ReferencesGrozinger C.M., Proc. Natl. Acad. Sci. U.S.A. 96:4868-4873(1999).
Amann J.M., Mol. Cell. Biol. 21:6470-6483(2001).
Gao L., J. Biol. Chem. 277:25748-25755(2002).
Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemistry analysis of paraffin-embedded human testis tissue using HDAC6 (Phospho-Ser22) antibody.
Immunohistochemistry analysis of paraffin-embedded human testis tissue using HDAC6 (Phospho-Ser22) antibody.
Western blot analysis of extracts from NIH/3T3 cells, treated with Anisomycin (25μg/ml, 30mins), using HDAC6 (Phospho-Ser22) antibody.
Western blot analysis of extracts from NIH/3T3 cells, treated with Anisomycin (25μg/ml, 30mins), using HDAC6 (Phospho-Ser22) antibody.
Immunofluorescence analysis of HepG2 cells, using HDAC6 (Phospho-Ser22) antibody.
Immunofluorescence analysis of HepG2 cells, using HDAC6 (Phospho-Ser22) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0941A-Conjugate. Price: $485 / Size: 100μl.

E11-0941A-FITC$485 / 100μlE11-0941A-Biotin$485 / 100μlE11-0941A-HRP$485 / 100μlE11-0941A-AP$485 / 100μlE11-0941A-AF350$485 / 100μlE11-0941A-AF405M$485 / 100μlE11-0941A-AF488$485 / 100μlE11-0941A-AF514$485 / 100μlE11-0941A-AF532$485 / 100μlE11-0941A-AF555$485 / 100μlE11-0941A-AF568$485 / 100μlE11-0941A-AF405S$485 / 100μlE11-0941A-AF405L$485 / 100μlE11-0941A-AF546$485 / 100μlE11-0941A-AF594$485 / 100μlE11-0941A-AF610$485 / 100μlE11-0941A-AF635$485 / 100μlE11-0941A-AF647$485 / 100μlE11-0941A-AF680$485 / 100μlE11-0941A-AF700$485 / 100μlE11-0941A-AF750$485 / 100μlE11-0941A-AF790$485 / 100μlE11-0941A-APC$485 / 100μlE11-0941A-PE$485 / 100μlE11-0941A-Cy3$485 / 100μlE11-0941A-Cy5$485 / 100μlE11-0941A-Cy5.5$485 / 100μlE11-0941A-Cy7$485 / 100μlE11-0941A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.