Other Names B-cell/myeloid kinase, BMK, EC 2.7.10.2, Hemopoietic cell kinase, P56-HCK and P60-HCK, Tyrosine-protein kinase HCK, kinase Hck, p59-HCK/p60-HCK
All Sites Human: Tyr410; Mouse: Tyr408; Rat: Tyr409
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human HCK around the phosphorylation site of tyrosine 410 (N-E-YP-T-A).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity HCK (Phospho-Tyr410) antibody detects endogenous levels of HCK only when phosphorylated at tyrosine 410.
ReferencesOta T., Nat. Genet. 36:40-45(2004).
Deloukas P., Nature 414:865-871(2001).
Olsen J.V., Cell 127:635-648(2006).