Other Names EC 2.7.10.2, FADK 1, FAK1, Focal adhesion kinase 1, PTK2, Protein- tyrosine kinase 2, pp125FAK
All Sites Human: Tyr397; Mouse: Tyr428; Rat: Tyr397
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human FAK around the phosphorylation site of tyrosine 397 (D-D-YP-A-E).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity FAK (Phospho-Tyr397) antibody detects endogenous levels of FAK only when phosphorylated at tyrosine 397.
ReferencesWhitney G.S., DNA Cell Biol. 12:823-830(1993).
Andre E., Biochem. Biophys. Res. Commun. 190:140-147(1993).
Lee S.-T., Oncogene 8:3403-3410(1993).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells