Other Names EC 2.7.11.24, Extracellular signal-regulated kinase 8
All Sites Human: Thr175+Tyr177; Mouse: Thr176+Tyr178
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human ERK8 around the phosphorylation site of threonine 175 and tyrosine 177 (A-V-TP-E-YP-V-A).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity ERK8 (Phospho-Thr175+Tyr177) antibody detects endogenous levels of ERK8 only when phosphorylated at threonine 175 and tyrosine 177.
ReferencesWen-Liang Kuo. J. Biol. Chem., May 2004; 279: 23073 - 23081.
Lorin M. Henrich. Mol. Cell. Biol., Sep 2003; 23: 5979 - 5988.
Zhijian Qian. J. Biol. Chem., Jan 1999; 274: 1621.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells