Other Names C-erbB-2, EC 2.7.10.1, Epidermal growth factor receptor-related protein, ErbB2, HER2, MLN 19, NEU, NEU proto-oncogene, NGL, Receptor protein-tyrosine kinase erbB-2, Receptor protein-tyrosine kinase erbB-2 precursor, Tyrosine kinase-type cell surface receptor HER2, kinase ErbB2, p185erbB2
All Sites Human: Thr686; Mouse: Thr736; Rat: Thr688
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human ErbB2 around the phosphorylation site of threonine 686(K-Y-TP-M-R).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity ErbB2 (Phospho-Thr686) antibody detects endogenous levels of ErbB2 only when phosphorylated at threonine 686.
ReferencesYamamoto T., Nature 319:230-234(1986).
King C.R., Science 229:974-976(1985).
Arcaro A., Mol. Cell. Biol. 20:3817-3830(2000).
Olsen J.V., Cell 127:635-648(2006).