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ErbB2 (Phospho-Thr686) Antibody
Datasheet
Catalog Number:
E11-0918AAmount:
100μgSwiss-Prot No.:
P04626Other Names:
C-erbB-2, EC 2.7.10.1, Epidermal growth factor receptor-related protein, ErbB2, HER2, MLN 19, NEU, NEU proto-oncogene, NGL, Receptor protein-tyrosine kinase erbB-2, Receptor protein-tyrosine kinase erbB-2 precursor, Tyrosine kinase-type cell surface receptor HER2, kinase ErbB2, p185erbB2All Sites:
Human: Thr686; Mouse: Thr736; Rat: Thr688Storage/Stability:
Store at -20℃/1 yearForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human ErbB2 around the phosphorylation site of threonine 686(K-Y-TP-M-R).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
ErbB2 (Phospho-Thr686) antibody detects endogenous levels of ErbB2 only when phosphorylated at threonine 686.Reactivity:
Human, Mouse, RatApplications:
IF: 1:100~1:500 ELISA: 1:5000References:
Yamamoto T., Nature 319:230-234(1986).
King C.R., Science 229:974-976(1985).
Arcaro A., Mol. Cell. Biol. 20:3817-3830(2000).
Olsen J.V., Cell 127:635-648(2006).
- P-peptide - +
Immunofluorescence analysis of HuvEc cells, using ErbB2 (Phospho-Thr686) antibody. Price:
225$
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