Other Names EC 2.7.10.1, ELK, EPB1, EPHT2, EPTH2, Eph receptor B1, Ephrin type-B receptor 1 precursor, HEK6, NET, Tyrosine-protein kinase receptor EPH-2, kinase EphB1
All Sites Human: Tyr594/596; Mouse: Tyr594/604; Rat: Tyr594
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human EPHB1/2 around the phosphorylation site of tyrosine 594/604 (K-I-YP-I-D).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity EPHB1/2 (Phospho-Tyr594/604) antibody detects endogenous levels of EPHB1/2 only when phosphorylated at s tyrosine 594/604.
ReferencesTang X.X., Genomics 29:426-437(1995).
Zhang Z., Protein Sci. 13:2819-2824(2004).
Stein E., J. Biol. Chem. 271:23588-23593(1996).