Other Names EC 2.7.10.1, ECK, Ephrin type-A receptor 2 precursor, Epithelial cell kinase, MPK-5, SEK-2, SEK2, Tyrosine-protein kinase receptor ECK, kinase EphA2
All Sites Human: Tyr588/Tyr596/Tyr596; Mouse: Tyr589/Tyr596/Tyr596
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human EPHA2/3/4 around the phosphorylation site of tyrosine 588/596 (T-YP-V-D-P).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity EPHA2/3/4 (Phospho-Tyr588/596) antibody detects endogenous levels of EPHA2/3/4 only when phosphorylated at tyrosine 588/596.
ReferencesLindberg R.A., Mol. Cell. Biol. 10:6316-6324(1990).
Zhang Y., Mol. Cell. Proteomics 4:1240-1250(2005).
Zhuang G., J. Biol. Chem. 282:2683-2694(2007).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells