Other Names DNA- PKcs, DNA-dependent protein kinase catalytic subunit, DNPK1, EC 2.7.11.1, P460, PRKD, PRKDC, XRCC7, kinase DNA-PK
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human DNA-PK around the phosphorylation site of threonine2647 (T-L-TP-Q-T).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity DNA-PK (Phospho-Thr2647) antibody detects endogenous levels of DNA-PK only when phosphorylated at threonine 2647.
ReferencesChung J., Korean J. Biochem. 27:193-197(1995).
Owsianka A.M., Lahn B.T., Virology 257:330-340(1999).
Lahn B.T., Science 278:675-680(1997).
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Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells