Other Names D1PAS1 related sequence 2, D1PAS1-RS2, DBX, DDX3X, DEAD-box RNA helicase DEAD3, DEAD-box protein 3, DEAD-box, X isoform, DEAD3, EC 3.6.1.-, ERH, Embryonic RNA helicase, HLP2, Helicase-like protein 2
All Sites Human: Thr322; Mouse: Thr322; Rat: Thr323
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human DDX3/DEAD-box Protein 3 around the phosphorylation site of threonine 322 (V-A-TP-P-G).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity DDX3/DEAD-box Protein 3 (Phospho-Thr322) antibody detects endogenous levels of DDX3/DEAD-box Protein 3 only when phosphorylated at threonine 322.
ReferencesChung J., Korean J. Biochem. 27:193-197(1995).
Owsianka A.M., Virology 257:330-340(1999).
Gevaert K., Proteomics 5:3589-3599(2005).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells