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DAPP1 (Phospho-Tyr139) Antibody
Datasheet
Catalog Number:
E11-0901AAmount:
100μgSwiss-Prot No.:
Q9UHF2Other Names:
3' phosphoinositide-interacting SH2 domain containing protein, B lymphocyte adapter protein BAM32, Bam32, Dual adaptor for phosphotyrosine and 3-phosphoinositides 1, PHISH, dual adapter for phosphotyrosine and 3-phosphoinositidesAll Sites:
Human: Tyr139; Mouse: Tyr139Storage/Stability:
Store at -20℃/1 yearForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human DAPP1 around the phosphorylation site of tyrosine 139 (S-I-YP-E-S).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
DAPP1 (Phospho-Tyr139) antibody detects endogenous levels of DAPP1 only when phosphorylated at tyrosine 139.Reactivity:
Human, MouseApplications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000References:
Dowler S., Biochem. J. 342:7-12(1999).
Marshall A.J., J. Exp. Med. 191:1319-1332(2000).
Zhang Q.-H., Genome Res. 10:1546-1560(2000).
- P-peptide - +
Western blot analysis of extracts from 293 cells, treated with Insulin (0.01U/ml, 2mins), using DAPP1 (Phospho-Tyr139) antibody.
Immunofluorescence analysis of A549 cells, using DAPP1 (Phospho-Tyr139) antibody. Price:
225$
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