Other Names C-RAF, C-Raf, CRAF, EC 2.7.11.1, Protein kinase raf 1, RAF proto-oncogene serine/threonine-protein kinase, RAF-1, RAF1, Raf proto-oncogene serine/threonine-protein kinase, Raf-1, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1, kinase Raf1
All Sites Human: Ser43; Mouse: Ser43; Rat: Ser43
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human c-RAF around the phosphorylation site of serine 43(R-A-SP-D-D).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity c-RAF (Phospho-Ser43) antibody detects endogenous levels of c-RAF only when phosphorylated at serine 43.
ReferencesMorrison D.K., . Biol. Chem. 268:17309-17316(1993).
King A.J., Nature 406:439-439(2000).
Olsen J.V., Cell 127:635-648(2006). Matsuoka S., Science 316:1160-1166(2007).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells