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Primary Antibodies
53BP1 (Phospho-Ser25) Antibody
Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)
IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000
$225/ 100μg
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Other Names P531, TP53B, TP53BP1, TRP53BP1, Tumor suppressor p53-binding protein 1, p53-binding protein 1
All Sites Human: Ser25; Mouse: Ser25; Rat: Ser30
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human 53BP1 around the phosphorylation site of serine 25 (E-D-SP-Q-P).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity 53BP1 (Phospho-Ser25) antibody detects endogenous levels of 53BP1 only when phosphorylated at serine 25.
ReferencesIrene M. Ward. Mol. Cell. Biol., Nov 2005; 25: 10079 - 10086.
Fiona Pryde. J. Cell Sci., May 2005; 118: 2043 - 2055.
Julio C. Morales. J. Biol. Chem., Apr 2003; 278: 14971 - 14977. .
Fiona Pryde. J. Cell Sci., May 2005; 118: 2043 - 2055.
Julio C. Morales. J. Biol. Chem., Apr 2003; 278: 14971 - 14977. .
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using 53BP1 (Phospho- Ser25) antibody.
Immunofluorescence analysis of NIH/3T3 cells, using 53BP1 (Phospho-Ser25) antibody.
53BP1 (Phospho-Ser25) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.
Conjugated Antibody
This product is also available conjugated. Format: E11-0886A-Conjugate. Price: $485 / Size: 100μl.
E11-0886A-FITC$485 / 100μlE11-0886A-Biotin$485 / 100μlE11-0886A-HRP$485 / 100μlE11-0886A-AP$485 / 100μlE11-0886A-AF350$485 / 100μlE11-0886A-AF405M$485 / 100μlE11-0886A-AF488$485 / 100μlE11-0886A-AF514$485 / 100μlE11-0886A-AF532$485 / 100μlE11-0886A-AF555$485 / 100μlE11-0886A-AF568$485 / 100μlE11-0886A-AF405S$485 / 100μlE11-0886A-AF405L$485 / 100μlE11-0886A-AF546$485 / 100μlE11-0886A-AF594$485 / 100μlE11-0886A-AF610$485 / 100μlE11-0886A-AF635$485 / 100μlE11-0886A-AF647$485 / 100μlE11-0886A-AF680$485 / 100μlE11-0886A-AF700$485 / 100μlE11-0886A-AF750$485 / 100μlE11-0886A-AF790$485 / 100μlE11-0886A-APC$485 / 100μlE11-0886A-PE$485 / 100μlE11-0886A-Cy3$485 / 100μlE11-0886A-Cy5$485 / 100μlE11-0886A-Cy5.5$485 / 100μlE11-0886A-Cy7$485 / 100μlE11-0886A-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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