Other Names CK 8, CK8, CYK8, Cytokeratin endo A, K2C8, K8, KRT2-8, KRT8, Keratin, type II cytoskeletal 8, cytokeratin 8
All Sites Human: Ser431; Mouse: Ser436; Rat: Ser431
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Cytokeratin 8 around the phosphorylation site of serine 431 (L-T-SP-P-G).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Cytokeratin 8 (Phospho-Ser431) antibody detects endogenous levels of Cytokeratin 8 only when phosphorylated at serine 431.
ReferencesTA Hembrough, J. Cell Sci., Mar 1995; 108: 1071.
MB Omary, J. Cell Biol., May 1992; 117: 583.
Joachim Seipelt, J. Biol. Chem., Jun 2000; 275: 20084.
CF Chou, J. Biol. Chem., Feb 1992; 267: 3901 - 3906.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells