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Keratin 8 (Phospho-Ser432) Antibody
Datasheet
Catalog Number:
E11-0883AAmount:
100μgSwiss-Prot No.:
P05787Other Names:
CK 8, CK8, CYK8, Cytokeratin endo A, K2C8, K8, KRT2-8, KRT8, Keratin, type II cytoskeletal 8, cytokeratin 8All Sites:
Human: Ser431; Mouse: Ser436; Rat: Ser431Storage/Stability:
Store at -20℃/1 yearForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Cytokeratin 8 around the phosphorylation site of serine 431 (L-T-SP-P-G).Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
Cytokeratin 8 (Phospho-Ser431) antibody detects endogenous levels of Cytokeratin 8 only when phosphorylated at serine 431.Reactivity:
Human, Mouse, RatApplications:
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000References:
TA Hembrough, J. Cell Sci., Mar 1995; 108: 1071.
MB Omary, J. Cell Biol., May 1992; 117: 583.
Joachim Seipelt, J. Biol. Chem., Jun 2000; 275: 20084.
CF Chou, J. Biol. Chem., Feb 1992; 267: 3901 - 3906.
- P-peptide - +
- Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Cytokeratin 8 (Phospho-Ser431) antibody.
Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using Cytokeratin 8 (Phospho-Ser431) antibody. 
- P-peptide - +
Immunofluorescence analysis of HeLa cells, using Cytokeratin 8 (Phospho-Ser431) antibody. Price:
225$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
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