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Primary Antibodies

Keratin 8 (Phospho-Ser432) Antibody

E11-0883ACite ID: EN0017665DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
P05787
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names CK 8, CK8, CYK8, Cytokeratin endo A, K2C8, K8, KRT2-8, KRT8, Keratin, type II cytoskeletal 8, cytokeratin 8
All Sites Human: Ser431; Mouse: Ser436; Rat: Ser431
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Cytokeratin 8 around the phosphorylation site of serine 431 (L-T-SP-P-G).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity Cytokeratin 8 (Phospho-Ser431) antibody detects endogenous levels of Cytokeratin 8 only when phosphorylated at serine 431.
ReferencesTA Hembrough, J. Cell Sci., Mar 1995; 108: 1071.
MB Omary, J. Cell Biol., May 1992; 117: 583.
Joachim Seipelt, J. Biol. Chem., Jun 2000; 275: 20084.
CF Chou, J. Biol. Chem., Feb 1992; 267: 3901 - 3906.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Cytokeratin 8 (Phospho-Ser431) antibody.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Cytokeratin 8 (Phospho-Ser431) antibody.
Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using Cytokeratin 8 (Phospho-Ser431) antibody.
Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using Cytokeratin 8 (Phospho-Ser431) antibody.
Immunofluorescence analysis of HeLa cells, using Cytokeratin 8 (Phospho-Ser431) antibody.
Immunofluorescence analysis of HeLa cells, using Cytokeratin 8 (Phospho-Ser431) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0883A-Conjugate. Price: $485 / Size: 100μl.

E11-0883A-FITC$485 / 100μlE11-0883A-Biotin$485 / 100μlE11-0883A-HRP$485 / 100μlE11-0883A-AP$485 / 100μlE11-0883A-AF350$485 / 100μlE11-0883A-AF405M$485 / 100μlE11-0883A-AF488$485 / 100μlE11-0883A-AF514$485 / 100μlE11-0883A-AF532$485 / 100μlE11-0883A-AF555$485 / 100μlE11-0883A-AF568$485 / 100μlE11-0883A-AF405S$485 / 100μlE11-0883A-AF405L$485 / 100μlE11-0883A-AF546$485 / 100μlE11-0883A-AF594$485 / 100μlE11-0883A-AF610$485 / 100μlE11-0883A-AF635$485 / 100μlE11-0883A-AF647$485 / 100μlE11-0883A-AF680$485 / 100μlE11-0883A-AF700$485 / 100μlE11-0883A-AF750$485 / 100μlE11-0883A-AF790$485 / 100μlE11-0883A-APC$485 / 100μlE11-0883A-PE$485 / 100μlE11-0883A-Cy3$485 / 100μlE11-0883A-Cy5$485 / 100μlE11-0883A-Cy5.5$485 / 100μlE11-0883A-Cy7$485 / 100μlE11-0883A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.