Other Names CK 18, CK18, CYK18, Cytokeratin endo B, K18, K1C18, KERD, KRT1-18, KRT18, Keratin, Keratin D, Keratin, type I cytoskeletal 18, cytokeratin 18, type I cytoskeletal 18
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Cytokeratin 18 around the phosphorylation site of serine 52 (S-T-SP-F-R).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Cytokeratin 18 (Phospho-Ser52) antibody detects endogenous levels of Cytokeratin 18 only when phosphorylated at serine 52.
ReferencesOshima R.G., Differentiation 33:61-68(1986).
Ji H., Electrophoresis 18:605-613(1997).
Ku N.-O., J. Biol. Chem. 270:11820-11827(1995).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells