Other Names CHEK1, Chk1, EC 2.7.11.1, kinase Chk1
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of serine 301 (D-F-SP-P-V).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Chk1 (Phospho-Ser301) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 301.
ReferencesSanchez Y., Science 277:1497-1501(1997).
Shieh S.-Y., Genes Dev. 14:289-300(2000).
Uensal-Kacmaz K., Mol. Cell. Biol. 25:3109-3116(2005).
Matsuoka S., Science 316:1160-1166(2007).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells