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Primary Antibodies

Chk1 (Phospho-Ser286) Antibody

E11-0861ACite ID: EN0017634DatasheetCOAMSDS
Human
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000
O14757
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names CHEK1, Chk1, EC 2.7.11.1, kinase Chk1
All Sites Human: Ser286
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of serine 286 (S-E-SP-P-S).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity Chk1 (Phospho-Ser286) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 286.
ReferencesSanchez Y., Science 277:1497-1501(1997).
Soerensen C.S., Cancer Cell 3:247-258(2003).
Matsuoka S., Science 316:1160-1166(2007).
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from K562 cells, treated with Na3VO4 (0.3uM, 40mins), using Chk1 (Phospho- Ser286) antibody.
Western blot analysis of extracts from K562 cells, treated with Na3VO4 (0.3uM, 40mins), using Chk1 (Phospho- Ser286) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0861A-Conjugate. Price: $485 / Size: 100μl.

E11-0861A-FITC$485 / 100μlE11-0861A-Biotin$485 / 100μlE11-0861A-HRP$485 / 100μlE11-0861A-AP$485 / 100μlE11-0861A-AF350$485 / 100μlE11-0861A-AF405M$485 / 100μlE11-0861A-AF488$485 / 100μlE11-0861A-AF514$485 / 100μlE11-0861A-AF532$485 / 100μlE11-0861A-AF555$485 / 100μlE11-0861A-AF568$485 / 100μlE11-0861A-AF405S$485 / 100μlE11-0861A-AF405L$485 / 100μlE11-0861A-AF546$485 / 100μlE11-0861A-AF594$485 / 100μlE11-0861A-AF610$485 / 100μlE11-0861A-AF635$485 / 100μlE11-0861A-AF647$485 / 100μlE11-0861A-AF680$485 / 100μlE11-0861A-AF700$485 / 100μlE11-0861A-AF750$485 / 100μlE11-0861A-AF790$485 / 100μlE11-0861A-APC$485 / 100μlE11-0861A-PE$485 / 100μlE11-0861A-Cy3$485 / 100μlE11-0861A-Cy5$485 / 100μlE11-0861A-Cy5.5$485 / 100μlE11-0861A-Cy7$485 / 100μlE11-0861A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.