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Primary Antibodies

CDC25B (Phospho-Ser353) Antibody

E11-0855ACite ID: EN0017626DatasheetCOAMSDS
Human
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
P30305
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names CDC25HU2, CDC25M2, Dual specificity phosphatase Cdc25B, EC 3.1.3.48, M-phase inducer phosphatase 2, MPIP2
All Sites Human: Ser353
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human CDC25B around the phosphorylation site of serine 353 (R-R-SP-V-T).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity CDC25B (Phospho-Ser353) antibody detects endogenous levels of CDC25B only when phosphorylated at serine 353.
ReferencesSanae Uchida, J. Cell Sci., Jun 2004; 117: 3011 - 3020.
Christina Karlsson, J. Cell Biol., Aug 1999; 146: 573.
Stéphanie Dutertre, J. Cell Sci., May 2004; 117: 2523 - 2531.
Price225$
Immunohistochemistry analysis of paraffin-embedded human brain tissue using CDC25B (Phospho-Ser353) antibody.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using CDC25B (Phospho-Ser353) antibody.
Western blot analysis of extracts from COS7 cells, treated with etoposide (25uM, 24hours), using CDC25B (Phospho-Ser353) antibody.
Western blot analysis of extracts from COS7 cells, treated with etoposide (25uM, 24hours), using CDC25B (Phospho-Ser353) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0855A-Conjugate. Price: $485 / Size: 100μl.

E11-0855A-FITC$485 / 100μlE11-0855A-Biotin$485 / 100μlE11-0855A-HRP$485 / 100μlE11-0855A-AP$485 / 100μlE11-0855A-AF350$485 / 100μlE11-0855A-AF405M$485 / 100μlE11-0855A-AF488$485 / 100μlE11-0855A-AF514$485 / 100μlE11-0855A-AF532$485 / 100μlE11-0855A-AF555$485 / 100μlE11-0855A-AF568$485 / 100μlE11-0855A-AF405S$485 / 100μlE11-0855A-AF405L$485 / 100μlE11-0855A-AF546$485 / 100μlE11-0855A-AF594$485 / 100μlE11-0855A-AF610$485 / 100μlE11-0855A-AF635$485 / 100μlE11-0855A-AF647$485 / 100μlE11-0855A-AF680$485 / 100μlE11-0855A-AF700$485 / 100μlE11-0855A-AF750$485 / 100μlE11-0855A-AF790$485 / 100μlE11-0855A-APC$485 / 100μlE11-0855A-PE$485 / 100μlE11-0855A-Cy3$485 / 100μlE11-0855A-Cy5$485 / 100μlE11-0855A-Cy5.5$485 / 100μlE11-0855A-Cy7$485 / 100μlE11-0855A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.