Other Names CDC25HU2, CDC25M2, Dual specificity phosphatase Cdc25B, EC 3.1.3.48, M-phase inducer phosphatase 2, MPIP2
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human CDC25B around the phosphorylation site of serine 353 (R-R-SP-V-T).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity CDC25B (Phospho-Ser353) antibody detects endogenous levels of CDC25B only when phosphorylated at serine 353.
ReferencesSanae Uchida, J. Cell Sci., Jun 2004; 117: 3011 - 3020.
Christina Karlsson, J. Cell Biol., Aug 1999; 146: 573.
Stéphanie Dutertre, J. Cell Sci., May 2004; 117: 2523 - 2531.