Other Names CD50 antigen; CDw50; ICA3; ICAM-R; ICAM3; Intercellular adhesion molecule-3 precursor
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human CD50/ICAM-3 around the phosphorylation site of serine 518 (S-G-SP-Y-H).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity CD50/ICAM-3 (Phospho-Ser518) antibody detects endogenous levels of CD50/ICAM-3 only when phosphorylated at serine 518.
ReferencesFawcett J., Nature 360:481-484(1992).
Vazeux R., Nature 360:485-488(1992).
de Fougerolles A.R., J. Exp. Med. 177:1187-1192(1993).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells