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CD5 (Phospho-Tyr453) Antibody
Datasheet
Catalog Number:
E11-0849AAmount:
100μgSwiss-Prot No.:
P06127Other Names:
LEU1, Lymphocyte antigen CD5, Lymphocyte glycoprotein Ly-1, Lymphocyte glycoprotein T1/Leu-1, Lyt-1, T-cell surface glycoprotein CD5 precursorAll Sites:
Human: Tyr453; Mouse: Tyr452Storage/Stability:
Store at -20℃/1 yearForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human CD5 around the phosphorylation site of tyrosine 453 (N-E-YP-S-Q).Puri-fication: The antibody was affinity-purified from
rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
CD5 (Phospho-Tyr453) antibody detects endogenous levels of CD5 only when phosphorylated at tyrosine 453.Reactivity:
Human, MouseApplications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000References:
Masanori Taniguchi, Blood, Feb 1998; 91: 1145 - 1151.
David Kaplan, PNAS, Nov 2001; 98: 13850.
SB Ebeling, J. Immunol., Dec 1993; 151: 6891.
Glenn H. Cantor, J. Virol., Feb 2001; 75: 1689 - 1696.
- P-peptide - +
Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using CD5 (Phospho-Tyr453) antibody.
Immunofluorescence analysis of HepG2 cells, using CD5 (Phospho-Tyr453) antibody. Price:
225$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
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