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CD45 (Phospho-Ser1007) Antibody
Datasheet
Catalog Number:
E11-0848AAmount:
100μgSwiss-Prot No.:
P08575Other Names:
EC 3.1.3.48, L-CA, LY-5, Leukocyte common antigen precursor, Leukocyte common antigen variant 4 precursor, Lymphocyte common antigen Ly-5, PTPRC, T200All Sites:
Human: Ser1007; Mouse: Ser996; Rat: Ser960Storage/Stability:
Store at -20℃/1 yearForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human CD45 around the phosphorylation site of serine 1007 (D-D-SP-D-S).Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
CD45 (Phospho-Ser1007) antibody detects endogenous levels of CD45 only when phosphorylated at serine 1007.Reactivity:
Human, Mouse, RatApplications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000References:
Streuli M., O J. 9:2399-2407(1990).
Wu L., Mol. Cell. Biol. 22:2673-2686(2002).
Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
- Western blot analysis of extracts from HeLa cells, treated with TNF (20ng/ml, 15mins), using CD45 (Phospho-Ser1007) antibody.
Price:
225$
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Catalog Number
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