Other Names T-cell-specific surface glycoprotein CD28 precursor, TP44
All Sites Human: Tyr218; Mouse: Tyr216
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human CD28 around the phosphorylation site of tyrosine 218 (A-A-YP-R-S).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity CD28 (Phospho-Tyr218) antibody detects endogenous levels of CD28 only when phosphorylated at tyrosine 218.
ReferencesAruffo A., Proc. Natl. Acad. Sci. U.S.A. 84:8573-8577(1987).
Lee K.P., J. Immunol. 145:344-352(1990).
Deshpande M., Hum. Immunol. 63:20-23(2002).
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Research AreaAutophagy antibody Cardiovascular Cancer Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells