Other Names Breast carcinoma-associated antigen DF3, CD227, Carcinoma-associated mucin, EMA, Episialin, H23AG, MUC-1, MUC1, Mucin 1 precursor, PEM, PEMT, PUM, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin
All Sites Human: Tyr1243; Mouse: Tyr618
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human CD227/Mucin 1 around the phosphorylation site oftyrosine 1243 (L-S-YP-T-N).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity CD227/Mucin 1 (Phospho-Tyr1243) antibody detects endogenous levels of CD227/Mucin 1 only when phosphorylated at tyrosine 1243.
ReferencesLan M.S., J. Biol. Chem. 265:15294-15299(1990).
Weiss M., Int. J. Cancer 66:55-59(1996).
Yu C.J., Oncology 53:118-126(1996).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells