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Primary Antibodies

Caspase 3 (Phospho-Ser150) Antibody

E11-0835ACite ID: EN0017591DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
P42574
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names Apopain precursor, CASP-3, CPP-32, CPP32, Caspase-3, Cysteine protease CPP32, EC 3.4.22.-, ICE3, SCA-1, SREBP cleavage activity 1, Yama protein
All Sites Human: Ser150; Mouse: Ser150; Rat: Ser150
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Caspase 3 around the phosphorylation site of serine 150 (C-R-SP-L-T).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity Caspase 3 (Phospho-Ser150) antibody detects endogenous levels of Caspase 3 only when phosphorylated at serine 150.
ReferencesDaniela Sciaky, J. Immunol., Apr 2000; 164: 3806.
Randolph Hutter, Circulation, Apr 2004; 109: 2001 - 2008.
Andreas Hartmann, PNAS, Mar 2000; 97: 2875.
Keith F. Izban, Am. J. Pathol., May 1999; 154: 1439.
Price225$
Immunohistochemistry analysis of paraffin-embedded h uman lymph node tissue using Caspase 3 (Phospho- Ser150) antibody.
Immunohistochemistry analysis of paraffin-embedded h uman lymph node tissue using Caspase 3 (Phospho- Ser150) antibody.
Western blot analysis of extracts from Jurkat cells, treated with Etoposide (25μM, 60mins), using Caspase 3 (Phospho-Ser150) antibody.
Western blot analysis of extracts from Jurkat cells, treated with Etoposide (25μM, 60mins), using Caspase 3 (Phospho-Ser150) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0835A-Conjugate. Price: $485 / Size: 100μl.

E11-0835A-FITC$485 / 100μlE11-0835A-Biotin$485 / 100μlE11-0835A-HRP$485 / 100μlE11-0835A-AP$485 / 100μlE11-0835A-AF350$485 / 100μlE11-0835A-AF405M$485 / 100μlE11-0835A-AF488$485 / 100μlE11-0835A-AF514$485 / 100μlE11-0835A-AF532$485 / 100μlE11-0835A-AF555$485 / 100μlE11-0835A-AF568$485 / 100μlE11-0835A-AF405S$485 / 100μlE11-0835A-AF405L$485 / 100μlE11-0835A-AF546$485 / 100μlE11-0835A-AF594$485 / 100μlE11-0835A-AF610$485 / 100μlE11-0835A-AF635$485 / 100μlE11-0835A-AF647$485 / 100μlE11-0835A-AF680$485 / 100μlE11-0835A-AF700$485 / 100μlE11-0835A-AF750$485 / 100μlE11-0835A-AF790$485 / 100μlE11-0835A-APC$485 / 100μlE11-0835A-PE$485 / 100μlE11-0835A-Cy3$485 / 100μlE11-0835A-Cy5$485 / 100μlE11-0835A-Cy5.5$485 / 100μlE11-0835A-Cy7$485 / 100μlE11-0835A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.