Other Names APAF-3, Apoptotic protease Mch-6, Apoptotic protease activating factor 3, CASP-9, Caspase-9, Caspase-9 precursor, Caspase9, EC 3.4.22.-, ICE-LAP6, ICE-like apoptotic protease 6, MCH6, RNCASP9
All Sites Human: Tyr153; Mouse: Tyr191; Rat: Tyr191
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Caspase 9 around the phosphorylation site of tyrosine 153 (L-A-YP-I-L).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Caspase 9 (Phospho-Tyr153) antibody detects endogenous levels of Caspase 9 only when phosphorylated at tyrosine 153.
ReferencesDuan H., J. Biol. Chem. 271:16720-16724(1996).
Srinivasula S.M., J. Biol. Chem. 271:27099-27106(1996).
Hadano S., Mamm. Genome 10:757-760(1999).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells