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Primary Antibodies

Caspase 9 (Phospho-Tyr153) Antibody

E11-0832ACite ID: EN0017585DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000
P55211
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names APAF-3, Apoptotic protease Mch-6, Apoptotic protease activating factor 3, CASP-9, Caspase-9, Caspase-9 precursor, Caspase9, EC 3.4.22.-, ICE-LAP6, ICE-like apoptotic protease 6, MCH6, RNCASP9
All Sites Human: Tyr153; Mouse: Tyr191; Rat: Tyr191
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Caspase 9 around the phosphorylation site of tyrosine 153 (L-A-YP-I-L).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity Caspase 9 (Phospho-Tyr153) antibody detects endogenous levels of Caspase 9 only when phosphorylated at tyrosine 153.
ReferencesDuan H., J. Biol. Chem. 271:16720-16724(1996).
Srinivasula S.M., J. Biol. Chem. 271:27099-27106(1996).
Hadano S., Mamm. Genome 10:757-760(1999).
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from COLO205 cells, using Caspase 9 (Phospho-Tyr153) antibody.
Western blot analysis of extracts from COLO205 cells, using Caspase 9 (Phospho-Tyr153) antibody.
Immunofluorescence analysis of HeLa cells, using Caspase 9 (Phospho-Tyr153) antibody.
Immunofluorescence analysis of HeLa cells, using Caspase 9 (Phospho-Tyr153) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0832A-Conjugate. Price: $485 / Size: 100μl.

E11-0832A-FITC$485 / 100μlE11-0832A-Biotin$485 / 100μlE11-0832A-HRP$485 / 100μlE11-0832A-AP$485 / 100μlE11-0832A-AF350$485 / 100μlE11-0832A-AF405M$485 / 100μlE11-0832A-AF488$485 / 100μlE11-0832A-AF514$485 / 100μlE11-0832A-AF532$485 / 100μlE11-0832A-AF555$485 / 100μlE11-0832A-AF568$485 / 100μlE11-0832A-AF405S$485 / 100μlE11-0832A-AF405L$485 / 100μlE11-0832A-AF546$485 / 100μlE11-0832A-AF594$485 / 100μlE11-0832A-AF610$485 / 100μlE11-0832A-AF635$485 / 100μlE11-0832A-AF647$485 / 100μlE11-0832A-AF680$485 / 100μlE11-0832A-AF700$485 / 100μlE11-0832A-AF750$485 / 100μlE11-0832A-AF790$485 / 100μlE11-0832A-APC$485 / 100μlE11-0832A-PE$485 / 100μlE11-0832A-Cy3$485 / 100μlE11-0832A-Cy5$485 / 100μlE11-0832A-Cy5.5$485 / 100μlE11-0832A-Cy7$485 / 100μlE11-0832A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.