Other Names AKT, AKT1 kinase, C-AKT, EC 2.7.11.1, PKB, PKB-alpha, Protein kinase B, RAC, RAC-PK-alpha, RAC-alpha serine/threonine kinase, kinase Akt1
All Sites Human: Tyr326; Mouse: Tyr326; Rat: Tyr326
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of tyrosine 326 (N-D-YP-G-R).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Akt (Phospho-Tyr326) antibody detects endogenous levels of Akt only when phosphorylated at tyrosine 326.
ReferencesJones P.F., Proc. Natl. Acad. Sci. U.S.A. 88:4171-4175(1991).
Matsubara A., Diabetologia 44:910-913(2001).
Olsen J.V., Cell 127:635-648(2006).
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Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells