Other Names EC 2.7.11.13; kinase PKC-theta; KPCT; nPKC-theta; PKC-theta; PKCQ; PRKCQ; PRKCT; Protein kinase C, theta type
All Sites Human: Ser695; Mouse: Ser695
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PKC θ around the phosphorylation site of serine 695 (N-F-SP-F-M).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity PKC θ (Phospho-Ser695) antibody detects endogenous levels of PKC θ only when phosphorylated at serine 695.
ReferencesChang J.D., J. Biol. Chem. 268:14208-14214(1993).
Chang J.D., J. Biol. Chem. 269:31322-31322(1994).
Baier G., J. Biol. Chem. 268:4997-5004(1993).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells