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Primary Antibodies
PKC ε (Phospho-Ser729) Antibody
$225/ 100μg
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Other Names EC 2.7.11.13, KPCE, PKC-epsilon, PKCEA, PRKCE, Protein kinase C, epsilon type, kinase PKC-epsilon, nPKC-epsilon
All Sites Human: Ser729; Mouse: Ser729; Rat: Ser729
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PKC ε around the phosphorylation site of serine 729 (G-F-SP-Y-F).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity PKC ε (Phospho-Ser729) antibody detects endogenous levels of PKC ε only when phosphorylated at serine 729.
ReferencesBasta P., Biochim. Biophys. Acta 1132:154-160(1992).
Hillier L.W., Nature 434:724-731(2005).
Cenni V., Biochem. J. 363:537-545(2002).
Hillier L.W., Nature 434:724-731(2005).
Cenni V., Biochem. J. 363:537-545(2002).
Price225$
Western blot analysis of extracts from HeLa cells (line 1), Jurkat cells (line 2) and NIH/3T3 cells (line 3) all treated with PMA (125ng/ml, 30mins), using PKC ε (Phospho- Ser729) antibody.
Conjugated Antibody
This product is also available conjugated. Format: E11-0802A-Conjugate. Price: $485 / Size: 100μl.
E11-0802A-FITC$485 / 100μlE11-0802A-Biotin$485 / 100μlE11-0802A-HRP$485 / 100μlE11-0802A-AP$485 / 100μlE11-0802A-AF350$485 / 100μlE11-0802A-AF405M$485 / 100μlE11-0802A-AF488$485 / 100μlE11-0802A-AF514$485 / 100μlE11-0802A-AF532$485 / 100μlE11-0802A-AF555$485 / 100μlE11-0802A-AF568$485 / 100μlE11-0802A-AF405S$485 / 100μlE11-0802A-AF405L$485 / 100μlE11-0802A-AF546$485 / 100μlE11-0802A-AF594$485 / 100μlE11-0802A-AF610$485 / 100μlE11-0802A-AF635$485 / 100μlE11-0802A-AF647$485 / 100μlE11-0802A-AF680$485 / 100μlE11-0802A-AF700$485 / 100μlE11-0802A-AF750$485 / 100μlE11-0802A-AF790$485 / 100μlE11-0802A-APC$485 / 100μlE11-0802A-PE$485 / 100μlE11-0802A-Cy3$485 / 100μlE11-0802A-Cy5$485 / 100μlE11-0802A-Cy5.5$485 / 100μlE11-0802A-Cy7$485 / 100μlE11-0802A-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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