Other Names Bloom's syndrome protein, DNA helicase, RecQ-like, type 2, EC 3.6.1.-, RECQ2, RECQL3, RecQ protein-like 3
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Bloom Syndrome Protein around the phosphorylation site of threonine 99 (Q-E-TP-Q-R).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Bloom Syndrome Protein (Phospho-Thr99) antibody detects endogenous levels of Bloom Syndrome Protein only when phosphorylated at threonine 99.
ReferencesEllis N.A., Cell 83:655-666(1995).
Karow J.K., J. Biol. Chem. 272:30611-30614(1997).
Pichierri P., EMBO J. 23:3154-3163(2004).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells