World supplier of antibodies, antigens and kits since 2007
Primary Antibodies
A-RAF (Phospho-Tyr302) Antibody
$225/ 100μg
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Other Names A-RAF, A-Raf proto-oncogene serine/threonine-protein kinase, A-raf-1, ARAF, ARAF1, EC 2.7.11.1, PKS, PKS2, Proto-oncogene Pks, Raf, kinase A-Raf
All Sites Human: Tyr301/Tyr302; Mouse: Tyr299/Tyr300; Rat: Tyr299/Tyr300
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human A-RAF around the phosphorylation site of tyrosine 301/tyrosine 302 (G-Y-YP-W-E).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity A-RAF (Phospho-Tyr301/302) antibody detects endogenous levels of A-RAF only when phosphorylated at tyrosine 301/tyrosine 302.
ReferencesOliver G. Rössler. Am J Physiol Cell Physiol, May 2004; 286: C1118 - C1129.
Marie A. Bogoyevitch. J. Biol. Chem., Nov 1995; 270: 26303.
Weicheng Liu. J. Biol. Chem., Mar 2004; 279: 10167 - 10175.
M Huleihel. Mol. Cell. Biol., Jul 1986; 6: 2655 - 2662.
Marie A. Bogoyevitch. J. Biol. Chem., Nov 1995; 270: 26303.
Weicheng Liu. J. Biol. Chem., Mar 2004; 279: 10167 - 10175.
M Huleihel. Mol. Cell. Biol., Jul 1986; 6: 2655 - 2662.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using A-RAF (Phospho- Tyr301/302) antibody.
Western blot analysis of extracts from HeLa cells, treated with PMA (125ng/ml, 30mins), using A-RAF (Phospho- Tyr301/302) antibody.
Immunofluorescence analysis of HepG2 cells, using A-RAF (Phospho-Tyr301/302) antibody.
Conjugated Antibody
This product is also available conjugated. Format: E11-0770A-Conjugate. Price: $485 / Size: 100μl.
E11-0770A-FITC$485 / 100μlE11-0770A-Biotin$485 / 100μlE11-0770A-HRP$485 / 100μlE11-0770A-AP$485 / 100μlE11-0770A-AF350$485 / 100μlE11-0770A-AF405M$485 / 100μlE11-0770A-AF488$485 / 100μlE11-0770A-AF514$485 / 100μlE11-0770A-AF532$485 / 100μlE11-0770A-AF555$485 / 100μlE11-0770A-AF568$485 / 100μlE11-0770A-AF405S$485 / 100μlE11-0770A-AF405L$485 / 100μlE11-0770A-AF546$485 / 100μlE11-0770A-AF594$485 / 100μlE11-0770A-AF610$485 / 100μlE11-0770A-AF635$485 / 100μlE11-0770A-AF647$485 / 100μlE11-0770A-AF680$485 / 100μlE11-0770A-AF700$485 / 100μlE11-0770A-AF750$485 / 100μlE11-0770A-AF790$485 / 100μlE11-0770A-APC$485 / 100μlE11-0770A-PE$485 / 100μlE11-0770A-Cy3$485 / 100μlE11-0770A-Cy5$485 / 100μlE11-0770A-Cy5.5$485 / 100μlE11-0770A-Cy7$485 / 100μlE11-0770A-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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