Other Names ADA17, ADAM 17, CD156b antigen, CSVP, EC 3.4.24.86, TACE, TNF-alpha convertase, TNF-alpha-converting enzyme, a disintegrin and metalloproteinase domain 17, snake venom-like protease
All Sites Human: Thr735; Mouse: Thr735; Rat: Thr735
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human ADAM 17 around the phosphorylation site of threonine 735 (P-Q-TP-P-G).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity ADAM 17 (Phospho-Thr735) antibody detects endogenous levels of ADAM 17 only when phosphorylated at threonine 735.
ReferencesMoss M.L., Nature 385:733-736(1997).
Patel I.R., J. Immunol. 160:4570-4579(1998).
Diaz-Rodriguez E., Mol. Biol. Cell 13:2031-2044(2002).
Olsen J.V., Cell 127:635-648(2006).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells