Other Names ACTA, ACTA1, ACTS, Actin, alpha skeletal muscle, Alpha-actin 1; ACTG, ACTG1, actin, cytoplasmic 2, gamma-actin
All Sites Human: Tyr55/Tyr53/Tyr53; Mouse: Tyr55/Tyr53/Tyr57
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Actin-pan (α/γ) around the phosphorylation site of tyrosine 53/55 (D-S-YP-V-G).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Actin-pan (α/γ) (Phospho-Tyr53/55) antibody detects endogenous levels of Actin-pan (α/γ) only when phosphorylated at tyrosine 53/55.
ReferencesA. J. MacQueen, Mol. Biol. Cell, Jul 2005; 16: 3247 - 3259.
Isaac Rabinovitz, J. Cell Biol., Dec 1997; 139: 1873.
Deanne H. Hryciw, Am J Physiol Renal Physiol, Jun 2005; 288: F1227 - F1235.
Derek T. Warren, J. Cell Sci., Apr 2002; 115: 1703.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells