Other Names 6-phosphofructo-2-kinase, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2, 6PF-2-K/Fru-2,6-P2ase heart-type isozyme, F262, PFK-2/FBPase-2, PFK-2/FBPase-2, heart type, fructose-2,6-bisphosphatase
All Sites Human: Ser483; Mouse: Ser486; Rat: Ser486
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PFKFB2 around the phosphorylation site of serine 483 (N-Y-SP-V-G).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity PFKFB2 (Phospho-Ser483) antibody detects endogenous levels of PFKFB2 only when phosphorylated at serine 483.
ReferencesSJ Pilkis, J. Biol. Chem., May 1983; 258: 6135 - 6141.
Tsuyoshi Furumoto, Plant Cell Physiol., Oct 2001; 42: 1044 - 1048.
AD Colosia, J. Biol. Chem., Dec 1988; 263: 18669 - 18677.
MR el-Maghrabi, J. Biol. Chem., Aug 1987; 262: 11714 - 11720.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells