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Primary Antibodies

PFKFB2 (Phospho-Ser483) Antibody

E11-0761ACite ID: EN0017471DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
O60825
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names 6-phosphofructo-2-kinase, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2, 6PF-2-K/Fru-2,6-P2ase heart-type isozyme, F262, PFK-2/FBPase-2, PFK-2/FBPase-2, heart type, fructose-2,6-bisphosphatase
All Sites Human: Ser483; Mouse: Ser486; Rat: Ser486
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PFKFB2 around the phosphorylation site of serine 483 (N-Y-SP-V-G).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity PFKFB2 (Phospho-Ser483) antibody detects endogenous levels of PFKFB2 only when phosphorylated at serine 483.
ReferencesSJ Pilkis, J. Biol. Chem., May 1983; 258: 6135 - 6141.
Tsuyoshi Furumoto, Plant Cell Physiol., Oct 2001; 42: 1044 - 1048.
AD Colosia, J. Biol. Chem., Dec 1988; 263: 18669 - 18677.
MR el-Maghrabi, J. Biol. Chem., Aug 1987; 262: 11714 - 11720.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PFKFB2 (Phospho- Ser483) antibody.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PFKFB2 (Phospho- Ser483) antibody.
Western blot analysis of extracts from 293 cells, treated with Heat shock, using PFKFB2 (Phospho-Ser483) antibody.
Western blot analysis of extracts from 293 cells, treated with Heat shock, using PFKFB2 (Phospho-Ser483) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0761A-Conjugate. Price: $485 / Size: 100μl.

E11-0761A-FITC$485 / 100μlE11-0761A-Biotin$485 / 100μlE11-0761A-HRP$485 / 100μlE11-0761A-AP$485 / 100μlE11-0761A-AF350$485 / 100μlE11-0761A-AF405M$485 / 100μlE11-0761A-AF488$485 / 100μlE11-0761A-AF514$485 / 100μlE11-0761A-AF532$485 / 100μlE11-0761A-AF555$485 / 100μlE11-0761A-AF568$485 / 100μlE11-0761A-AF405S$485 / 100μlE11-0761A-AF405L$485 / 100μlE11-0761A-AF546$485 / 100μlE11-0761A-AF594$485 / 100μlE11-0761A-AF610$485 / 100μlE11-0761A-AF635$485 / 100μlE11-0761A-AF647$485 / 100μlE11-0761A-AF680$485 / 100μlE11-0761A-AF700$485 / 100μlE11-0761A-AF750$485 / 100μlE11-0761A-AF790$485 / 100μlE11-0761A-APC$485 / 100μlE11-0761A-PE$485 / 100μlE11-0761A-Cy3$485 / 100μlE11-0761A-Cy5$485 / 100μlE11-0761A-Cy5.5$485 / 100μlE11-0761A-Cy7$485 / 100μlE11-0761A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.