Other Names 14-3-3 protein zeta/delta, 1433Z, 143Z, FAS, Factor activating exoenzyme S, KCIP-1, Mitochondrial import stimulation factor S1 subunit, Protein kinase C inhibitor protein-1, YWHAZ
All Sites Human: Thr232; Mouse: Thr232
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human 14-3-3 ζ/δ around the phosphorylation site of threonine 232 (S-D-TP-Q-G).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity 14-3-3 ζ/δ (Phospho-Thr232) antibody detects endogenous levels of 14-3-3 ζ/δ only when phosphorylated at threonine 232.
ReferencesAlastair Aitken, J. Biol. Chem., Mar 1995; 270: 5706.
L Kockel, Genes & Dev., May 1997; 11: 1140 - 1147.
Martijn J. van Hemert, J. Cell Sci., Mar 2004; 117: 1411 - 1420.
Anne-Sophie Vercoutter-Edouart, Cancer Res., Jan 2001; 61: 76 - 80.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells