Other Names 14-3-3 protein T-cell, 14-3-3 protein tau, 1433T, 143T, HS1 protein, YWHAQ
All Sites Human: Ser232; Mouse: Ser232
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human 14-3-3 θ/τ around the phosphorylation site of serine 232 (S-D-SP-A-G).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity 14-3-3 θ/τ (Phospho-Ser232) antibody detects endogenous levels of 14-3-3 θ/τ only when phosphorylated at serine 232.
ReferencesNielsen P.J., im. Biophys. Acta 1088:425-428(1991).
Leffers H., J. Mol. Biol. 231:982-998(1993).
Gevaert K., Nat. Biotechnol. 21:566-569(2003).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells