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Primary Antibodies

YAP (Phospho-Ser127) Antibody

E11-0757ACite ID: EN0017462DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
P46937
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names 65 kDa Yes-associated protein, YAP1, YAP65
All Sites Human: Ser127; Mouse: Ser112; Rat: Ser112
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human YAP around the phosphorylation site of serine 127 (A-H-SP-S-P).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity YAP (Phospho-Ser127) antibody detects endogenous levels of YAP only when phosphorylated at serine 127.
ReferencesSudol M., J. Biol. Chem. 270:14733-14741(1995).
Chen H.I., J. Biol. Chem. 272:17070-17077(1997).
Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Price225$
Immunohistochemistry analysis of paraffin-embedded human heart tissue using YAP (Phospho-Ser127) antibody.
Immunohistochemistry analysis of paraffin-embedded human heart tissue using YAP (Phospho-Ser127) antibody.
Western blot analysis of extracts from COS7 cells, treated with HU (2nM, 24hours), using YAP (Phospho-Ser127) antibody.
Western blot analysis of extracts from COS7 cells, treated with HU (2nM, 24hours), using YAP (Phospho-Ser127) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0757A-Conjugate. Price: $485 / Size: 100μl.

E11-0757A-FITC$485 / 100μlE11-0757A-Biotin$485 / 100μlE11-0757A-HRP$485 / 100μlE11-0757A-AP$485 / 100μlE11-0757A-AF350$485 / 100μlE11-0757A-AF405M$485 / 100μlE11-0757A-AF488$485 / 100μlE11-0757A-AF514$485 / 100μlE11-0757A-AF532$485 / 100μlE11-0757A-AF555$485 / 100μlE11-0757A-AF568$485 / 100μlE11-0757A-AF405S$485 / 100μlE11-0757A-AF405L$485 / 100μlE11-0757A-AF546$485 / 100μlE11-0757A-AF594$485 / 100μlE11-0757A-AF610$485 / 100μlE11-0757A-AF635$485 / 100μlE11-0757A-AF647$485 / 100μlE11-0757A-AF680$485 / 100μlE11-0757A-AF700$485 / 100μlE11-0757A-AF750$485 / 100μlE11-0757A-AF790$485 / 100μlE11-0757A-APC$485 / 100μlE11-0757A-PE$485 / 100μlE11-0757A-Cy3$485 / 100μlE11-0757A-Cy5$485 / 100μlE11-0757A-Cy5.5$485 / 100μlE11-0757A-Cy7$485 / 100μlE11-0757A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.