Other Names 65 kDa Yes-associated protein, YAP1, YAP65
All Sites Human: Ser127; Mouse: Ser112; Rat: Ser112
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human YAP around the phosphorylation site of serine 127 (A-H-SP-S-P).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity YAP (Phospho-Ser127) antibody detects endogenous levels of YAP only when phosphorylated at serine 127.
ReferencesSudol M., J. Biol. Chem. 270:14733-14741(1995).
Chen H.I., J. Biol. Chem. 272:17070-17077(1997).
Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).