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Primary Antibodies

PKD1/2/3/PKC μ (Phospho-Ser738+Ser742) Antibody

E11-0720ACite ID: EN0017440DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Q15139
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names EC 2.7.11.13, KPCD1, PKC-mu, PKCM, PKD, PRKCM, PRKD1, Protein kinase C, mu type, Protein kinase D, kinase PKD1, nPKC-mu
All Sites Human: Ser738+Ser742; Mouse: Ser744+Ser748; Rat: Ser475+Ser479
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PKD1/2/3/PKC μ around the phosphorylation site of serine 738and serine 742 (E-K-SP-F-R-R-SP-V-V).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity PKD1/2/3/PKC μ (Phospho-Ser738+Ser742) antibody detects endogenous levels of PKD1/2/3/PKC μ only when phosphorylated at serine 738and serine 742.
ReferencesJohannes F.-J., J. Biol. Chem. 269:6140-6148(1994).
Zemlickova E., Biochem. Biophys. Res. Commun. 307:459-465(2003).
Storz P., J. Biol. Chem. 278:17969-17976(2003).
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemistry analysis of paraffin-embedded human heart tissue using PKD1/2/3/PKC μ (Phospho- Ser738+Ser742) antibody.
Immunohistochemistry analysis of paraffin-embedded human heart tissue using PKD1/2/3/PKC μ (Phospho- Ser738+Ser742) antibody.
Western blot analysis of extracts from A549 cells treated with PMA (125ng/ml, 30mins), and HT-29 cells treated with serum (20%, 15mins), using PKD1/2/3/PKC μ (Phospho-Ser738+Ser742) antibody.
Western blot analysis of extracts from A549 cells treated with PMA (125ng/ml, 30mins), and HT-29 cells treated with serum (20%, 15mins), using PKD1/2/3/PKC μ (Phospho-Ser738+Ser742) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0720A-Conjugate. Price: $485 / Size: 100μl.

E11-0720A-FITC$485 / 100μlE11-0720A-Biotin$485 / 100μlE11-0720A-HRP$485 / 100μlE11-0720A-AP$485 / 100μlE11-0720A-AF350$485 / 100μlE11-0720A-AF405M$485 / 100μlE11-0720A-AF488$485 / 100μlE11-0720A-AF514$485 / 100μlE11-0720A-AF532$485 / 100μlE11-0720A-AF555$485 / 100μlE11-0720A-AF568$485 / 100μlE11-0720A-AF405S$485 / 100μlE11-0720A-AF405L$485 / 100μlE11-0720A-AF546$485 / 100μlE11-0720A-AF594$485 / 100μlE11-0720A-AF610$485 / 100μlE11-0720A-AF635$485 / 100μlE11-0720A-AF647$485 / 100μlE11-0720A-AF680$485 / 100μlE11-0720A-AF700$485 / 100μlE11-0720A-AF750$485 / 100μlE11-0720A-AF790$485 / 100μlE11-0720A-APC$485 / 100μlE11-0720A-PE$485 / 100μlE11-0720A-Cy3$485 / 100μlE11-0720A-Cy5$485 / 100μlE11-0720A-Cy5.5$485 / 100μlE11-0720A-Cy7$485 / 100μlE11-0720A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.