Other Names EC 2.7.11.13, KPCT, PKC-theta, PKCQ, PRKCQ, PRKCT, Protein kinase C, theta type, kinase PKC-theta, nPKC-theta
All Sites Human: Thr538; Mouse: Thr538
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PKC θ around the phosphorylation site of threonine 538 (T-N-TP-F-C).
Purification The antibody was affinity-purified frorabbitantiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity PKC θ (Phospho-Thr538) antibody detects endogenous levels of PKC θ only when phosphorylated at threonine 538.
ReferencesBaier G., J. Biol. Chem. 268:4997-5004(1993).
Deloukas P., Nature 429:375-381(2004).
Wissing J., Mol. Cell. Proteomics 6:537-547(2007).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells