Other Names EC 2.7.11.13, KPCD, PKC-delta, PRKCD, kinase PKC-delta, nPKC-delta
All Sites Human: Tyr313; Mouse: Tyr311; Rat: Tyr311
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PKC δ around the phosphorylation site of tyrosine 313 (G-I-YP-Q-G).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity PKC δ (Phospho-Tyr313) antibody detects endogenous levels of PKC δ only when phosphorylated at tyrosine 313.
ReferencesAris J.P., Biochim. Biophys. Acta 1174:171-181(1993).
Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Benes C.H., Cell 121:271-280(2005).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells