Other Names EC 2.7.11.13, KPCA, PKC III, PKC-A, PKC-III, PKC-alpha, PRKACA, PRKCA, Protein kinase C, alpha type, kinase PKC-alpha
All Sites Human: Thr638; Mouse: Thr638; Rat: Thr638
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PKC α/β2 around the phosphorylation site of threonine 638 (V-L-TP-P-P).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity PKC α (Phospho-Thr638) antibody detects endogenous levels of PKC α only when phosphorylated at threonine 638.
ReferencesMaria Nikodemova, J. Biol. Chem., May 2007; 282: 15208 - 15216.
Pedro J. Cejas, J. Biol. Chem., Aug 2005; 280: 28412 - 28423.
Dan Chen, J. Biol. Chem., Feb 2004; 279: 4829 - 4839.
Nicole R. Murray, J. Cell Biol., May 1999; 145: 699.