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Primary Antibodies

PKC α (Phospho-Thr638) Antibody

E11-0716ACite ID: EN0017433DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
P17252
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names EC 2.7.11.13, KPCA, PKC III, PKC-A, PKC-III, PKC-alpha, PRKACA, PRKCA, Protein kinase C, alpha type, kinase PKC-alpha
All Sites Human: Thr638; Mouse: Thr638; Rat: Thr638
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PKC α/β2 around the phosphorylation site of threonine 638 (V-L-TP-P-P).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity PKC α (Phospho-Thr638) antibody detects endogenous levels of PKC α only when phosphorylated at threonine 638.
ReferencesMaria Nikodemova, J. Biol. Chem., May 2007; 282: 15208 - 15216.
Pedro J. Cejas, J. Biol. Chem., Aug 2005; 280: 28412 - 28423.
Dan Chen, J. Biol. Chem., Feb 2004; 279: 4829 - 4839.
Nicole R. Murray, J. Cell Biol., May 1999; 145: 699.
Price225$
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PKC α (Phospho- Thr638) antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PKC α (Phospho- Thr638) antibody.
Western blot analysis of extracts from NIH/3T3 cells, treated with UV (15mins), using PKC α (Phospho-Thr638) antibody.
Western blot analysis of extracts from NIH/3T3 cells, treated with UV (15mins), using PKC α (Phospho-Thr638) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0716A-Conjugate. Price: $485 / Size: 100μl.

E11-0716A-FITC$485 / 100μlE11-0716A-Biotin$485 / 100μlE11-0716A-HRP$485 / 100μlE11-0716A-AP$485 / 100μlE11-0716A-AF350$485 / 100μlE11-0716A-AF405M$485 / 100μlE11-0716A-AF488$485 / 100μlE11-0716A-AF514$485 / 100μlE11-0716A-AF532$485 / 100μlE11-0716A-AF555$485 / 100μlE11-0716A-AF568$485 / 100μlE11-0716A-AF405S$485 / 100μlE11-0716A-AF405L$485 / 100μlE11-0716A-AF546$485 / 100μlE11-0716A-AF594$485 / 100μlE11-0716A-AF610$485 / 100μlE11-0716A-AF635$485 / 100μlE11-0716A-AF647$485 / 100μlE11-0716A-AF680$485 / 100μlE11-0716A-AF700$485 / 100μlE11-0716A-AF750$485 / 100μlE11-0716A-AF790$485 / 100μlE11-0716A-APC$485 / 100μlE11-0716A-PE$485 / 100μlE11-0716A-Cy3$485 / 100μlE11-0716A-Cy5$485 / 100μlE11-0716A-Cy5.5$485 / 100μlE11-0716A-Cy7$485 / 100μlE11-0716A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.