Other Names EC 2.7.11.1, PIM-1, Proto-oncogene serine/threonine-protein kinase pim-1
All Sites Human: Tyr309; Mouse: Tyr302; Rat: Tyr218
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Pim-1 around the phosphorylation site of tyrosine 309 (H-R-YP-H-G).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Pim-1 (Phospho-Tyr309) antibody detects endogenous levels of Pim-1 only when phosphorylated at tyrosine 309.
ReferencesSelten G., Cell 46:603-611(1986).
Saris C.J., EMBO J. 10:655-664(1991).
Maita H., Eur. J. Biochem. 267:5168-5178(2000).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells