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Primary Antibodies

Pim-1 (Phospho-Tyr309) Antibody

E11-0712ACite ID: EN0017429DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 ELISA: 1:40000
P06803
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names EC 2.7.11.1, PIM-1, Proto-oncogene serine/threonine-protein kinase pim-1
All Sites Human: Tyr309; Mouse: Tyr302; Rat: Tyr218
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Pim-1 around the phosphorylation site of tyrosine 309 (H-R-YP-H-G).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity Pim-1 (Phospho-Tyr309) antibody detects endogenous levels of Pim-1 only when phosphorylated at tyrosine 309.
ReferencesSelten G., Cell 46:603-611(1986).
Saris C.J., EMBO J. 10:655-664(1991).
Maita H., Eur. J. Biochem. 267:5168-5178(2000).
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from HUVEC cells, treated with PMA (125ng/ml, 30mins), using Pim-1 (Phospho-Tyr309) antibody.
Western blot analysis of extracts from HUVEC cells, treated with PMA (125ng/ml, 30mins), using Pim-1 (Phospho-Tyr309) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0712A-Conjugate. Price: $485 / Size: 100μl.

E11-0712A-FITC$485 / 100μlE11-0712A-Biotin$485 / 100μlE11-0712A-HRP$485 / 100μlE11-0712A-AP$485 / 100μlE11-0712A-AF350$485 / 100μlE11-0712A-AF405M$485 / 100μlE11-0712A-AF488$485 / 100μlE11-0712A-AF514$485 / 100μlE11-0712A-AF532$485 / 100μlE11-0712A-AF555$485 / 100μlE11-0712A-AF568$485 / 100μlE11-0712A-AF405S$485 / 100μlE11-0712A-AF405L$485 / 100μlE11-0712A-AF546$485 / 100μlE11-0712A-AF594$485 / 100μlE11-0712A-AF610$485 / 100μlE11-0712A-AF635$485 / 100μlE11-0712A-AF647$485 / 100μlE11-0712A-AF680$485 / 100μlE11-0712A-AF700$485 / 100μlE11-0712A-AF750$485 / 100μlE11-0712A-AF790$485 / 100μlE11-0712A-APC$485 / 100μlE11-0712A-PE$485 / 100μlE11-0712A-Cy3$485 / 100μlE11-0712A-Cy5$485 / 100μlE11-0712A-Cy5.5$485 / 100μlE11-0712A-Cy7$485 / 100μlE11-0712A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.