Other Names EC 2.7.12.2, MPAKK-like protein kinase cap site form 2, NORI-3, TOPK
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PBK/TOPK around the phosphorylation site of threonine 9 (F-K-TP-P-S).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity PBK/TOPK (Phospho-Thr9) antibody detects endogenous levels of PBK/TOPK only when phosphorylated at threonine 9.
ReferencesAbe Y., J. Biol. Chem. 275:21525-21531(2000).
Gaudet S., Proc. Natl. Acad. Sci. U.S.A. 97:5167-5172(2000).
Zhao S., Int. J. Biochem. Cell Biol. 33:631-636(2001).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells